Project

Part:BBa_K1728018:Design

Designed by: Yung-Li Chen, Katy Chang, Timothy Chan En Haw   Group: iGEM15_CGU_Taiwan   (2015-09-17)


DUSP1 toehold switch RNA sensor with T7 promoter & luciferase reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 47
    Illegal SapI.rc site found at 922


Design Notes

No.


Source

DUSP1 toehold switch RNA sensor with T7 promoter (order from synthesis company) assembled with luciferase reporter (BBa_I712019) at the upstream.

References

Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin Toehold Switches: De-Novo-Designed Regulators of Gene Expression Cell.2014;159:925-939